Metabolism of steroid estrogens in the hen IV. Conversion in vivo of estradiol-17β-6, 7- 3H-3, 17-disulfate to estradiol-17β-6, 7- 3H-17-sulfate

• Published in: Steroids Vol. 14; no. 4; pp. 389 - 402
• Main Authors: Mathur, R.S., Common, R.H., Hobkirk, R.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.10.1969
• ISSN: 0039-128X; 1878-5867
• DOI: 10.1016/S0039-128X(69)80002-4

Estradiol-17β-6, 7- 3H-3, 17-disulfate was injected intramuscularly into a mature non-laying hen. Very little (approximately 2%) of the injected material was recovered unchanged from the urine. The only radioactive metabolite identified in the urine was estradiol17β-17-sulfate. The identification of this compound is based on: (a) Mylase P enzyme incubations; (b) methylation followed by solvolysis and thin-layer chromatographic identification of estradiol-17β-3-methyl ether; (c) acetylation of the latter and t. l. c. identification of estradiol-17β-3-methyl ether-17-acetate; (d) crystallization with unlabelled estradiol-17β-17-sulfate; (e) solvolysis of the latter and crystallization with unlabelled estradiol-17β.