Challenges and perspectives in using unspecific peroxygenases for organic synthesis

In the past 20 years, unspecific peroxygenases (UPOs) have emerged as promising biocatalysts for various organic transformations. Particularly, we have witnessed great attention being paid to the screening of new enzymes and expansion of the substrates/products. However, challenges such as enzyme st...

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Bibliographic Details
Published inFrontiers in catalysis Vol. 4
Main Authors Huang, Yawen, Sha, Jiangtao, Zhang, Jie, Zhang, Wuyuan
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 15.10.2024
Subjects
biocatalysis
directed evolution
oxidoreductases
oxygenation
protein engineering
unspecific peroxygenases
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Summary:In the past 20 years, unspecific peroxygenases (UPOs) have emerged as promising biocatalysts for various organic transformations. Particularly, we have witnessed great attention being paid to the screening of new enzymes and expansion of the substrates/products. However, challenges such as enzyme stability, low turnover numbers, and substrate specificity hinder their widespread utilization in practical organic synthesis. This review article provides a concrete and mini-overview of the challenges associated with using UPOs in organic synthesis and discusses strategies for enzyme engineering to overcome these limitations. The article highlights recent advancements in UPO research and presents potential solutions to enhance their catalytic efficiency, stability, substrate specificity, and regioselectivity. Additionally, the review outlines the current methodologies employed for directed evolution and protein engineering of UPOs, along with computational modeling approaches for rational enzyme design. By addressing the challenges and exploring avenues for enzyme engineering, this review aims to shed light on the prospects of UPOs in organic synthesis.
ISSN:2673-7841
2673-7841
DOI:10.3389/fctls.2024.1470616