Testing of wheat to durable resistance and survey approach for Puccinia recondita tritici

• Published in: Genetika (Beograd) Vol. 40; no. 2; pp. 95 - 107
• Main Authors: Boskovic, Jelena, Boskovic, Momcilo, Prijic, Zeljana
• Format: Journal Article
• Language: English
• Published: 2008
• Subjects: Puccinia recondita tritici; Triticum aestivum
• ISSN: 0534-0012; 1820-6069
• DOI: 10.2298/GENSR0802095B

The main objective within new approach in international pathogenicity surveys of Puccinia recondita tritici was to provide genetically diverse sources of resistance (wheat lines with pyramiding resistant genes) to be used in a survey of wheat leaf rust pathogen in European-Mediterranean regions and to search for and document pathogenicity of P. recondita tritici cultures useful in differentiating sources of resistance. Emphasis is placed on sources of resistance and their usefulness rather than on description of fungus populations. In this international survey new methods have been applied containing Central Field Nursery, Central Seedling Tests, Cooperative Seedling Tests and Regional Field Nurseries (ELRWN-European Leaf Rust of Wheat Nursery). The results have been reported from one year of investigations. ELRWN contained 20 winter wheat hybrid lines with pyramiding resistant genes including strong ones Lr9, Lr19 and Lr24. In addition, 16 spring wheat lines were included, as control lines were Lr9, Lr18, Lr19, Lr24 and Lr14. In that year ELRWN have been realized in 13 countries and cooperative seedling test in 8 countries using 22 pathotypes of P. recondita tritici. The best results obtained by the winter wheat lines NS-66/5'Lr24, NS-77/2'Lr19, NS- 37/2'Lr19 and spring wheat lines 647-CMA-14793 and 26TH-ESWYT-10. The results have shown loosing almost complete resistance of Lr9 and Lr24, but much less Lr19. Glavni cilj u okviru novog pristupa medjunarodne analize populacije patogeniteta Puccinia recondita tritici sastojao se u ukljucenju geneticki razliciti izvora otpornosti (linije psenice sa akumulacijom gena otpornosti) koji su upotrebljeni u analizi populacije patogena lisne rdje psenice Evropsko-Mediteranskog podrucja kao i analiza dobijenih podataka patogeniteta kultura P. recondiat tritici korisnih u diferencijaciji izvora otpornosti. Istaknut je znacaj izvora otpornosti i njihova korisna primena pre nego opisivanje populacije. U ovim medjunarodnim analizama primenjene su nove metode u koje se ukljucuju testiranje centralnog poljskog rasadnika psenice, centralno testiranje sejanaca, kooperativna testiranja sejanaca i regionalni poljski rasadnici (ELRWN - Evropski Rasadnik za Patogena Lisne Rdje Psenice). Rezultati su saopsteni za jednogodisnja istrazivanja. ELRWN je sadrzavao 20 ozimih hibrida linija psenice sa akumulacijom gena otpornosti ukljucujuci jake gene Lr9, Lr19 i Lr24. Dodatno je ukljuceno 16 jarih linija psenice kao i kontrolne linije Lr9, Lr18, Lr19, Lr24 i Lr14.U toj godini rasadnik ELRWN je bio realizovan u 13 zemalja, a kooperativno testiranje sejanaca u 8 zemalja upotrebom 22 patotipa P. recondita tritici. Dobijeni su najbolji rezultati sa ozimim linijama psenice NS-66/5'Lr24, NS-77/2'Lr19, NS-37/2'Lr19 i jarim linijama psenice 647-CMA-14793 i 26TH-ESWYT-10. Ovi rezultati su pokazali gubitak skore potpune otpornosti linija Lr9 i Lr24, ali mnogo manje linije Lr19.