Production of Three Distinct mRNAs of 150 kDa Oxygen-Regulated Protein (ORP150) by Alternative Promoters: Preferential Induction of One Species under Stress Condition

150 kDa oxygen-regulated protein (ORP150) is one of the endoplasmic reticulum (ER)-resident stress proteins we have cloned and sequenced the entire human ORP150 gene covering over 15-kb. Analyses of transcription initiation sites and transcriptional regulatory sequences revealed that at least three...

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Published inJournal of biochemistry (Tokyo) Vol. 128; no. 3; pp. 529 - 538
Main Authors Kaneda, Sumiko, Yura, Takashi, Yanagi, Hideki
Format Journal Article
LanguageEnglish
Published Oxford University Press 01.09.2000
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Summary:150 kDa oxygen-regulated protein (ORP150) is one of the endoplasmic reticulum (ER)-resident stress proteins we have cloned and sequenced the entire human ORP150 gene covering over 15-kb. Analyses of transcription initiation sites and transcriptional regulatory sequences revealed that at least three distinct mRNA species were produced by alternative promoters: two of them starting from alternative exon 1 (1A or 1B), and the third one starting from exon 2, six nucleotides upstream of the first AUG initiation codon. Among them, the transcript that begins with exon 1B was preferentially induced by hypoxia or tunicamycin treatment. A cis-acting segment involved in the stress-dependent induction was found at the 5‘-end of exon 1A which could account for the selective induction of the transcription from exon 1B. Furthermore, in vitro analyses of translation of the thire mRNA suggested the constitutive expression of the cytosolic ORP150 due to the lack of the signal peptide resulting from differential translation initiation
Bibliography:1The nucleotide sequence reported in this paper has been submitted to the DDBJ under accession number AB009979
ArticleID:128.3.529
ark:/67375/HXZ-HG10XX0P-X
istex:C799ECBCD26AF5E75C40F611AF826685DB17BE27
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a022783