Flower color chimera and abnormal leaf mutants induced by ¹²C⁶⁺ heavy ions in Salvia splendens Ker-Gawl

• Published in: Scientia horticulturae Vol. 121; no. 4; pp. 462 - 467
• Main Authors: Wu, D.L, Hou, S.W, Qian, P.P, Sun, L.D, Zhang, Y.C, Li, W.J
• Format: Journal Article
• Language: English
• Published: Amsterdam [Amsterdam; New York, NY]: Elsevier Science 01.08.2009; Elsevier
• Subjects: Agronomy. Soil science and plant productions; appearance (quality); bedding plants; benzyladenine; Biological and medical sciences; cations; color; culture media; dose response; flowers; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; genetic variation; irradiation; leaves; micropropagation; mutagenesis; mutants; mutation; naphthaleneacetic acid; nursery crops; random amplified polymorphic DNA technique; Salvia splendens; Vegetative propagation. Micropropagation; Vegetative propagation. Sowing and planting. Harvesting; Flower; Horticulture; Leaf mutants; RAPD; Salvia splendens Ker-Gawl; Color; Ions; Plant leaf; Micropropagation; Random amplified polymorphic DNA marker; Labiatae; Dicotyledones; Angiospermae; Flower crop; Spermatophyta; Mutation; Flower color chimera; Salvia splendens; Chimera
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2009.02.022

The effect of ¹²C⁶⁺ heavy ions bombardment on mutagenesis in Salvia splendens Ker-Gawl. was studied. Dose-response studies indicated that there was a peak of malformation frequency of S. splendens at 200Gy. Abnormal leaf mutants of the bileaf, trileaf and tetraleaf conglutination were selected. Meanwhile, a bicolor flower chimera with dark red and fresh red flower was isolated in M1 generation of S. splendens. Random amplified polymorphic DNA (RAPD) analysis demonstrated that DNA variations existed among the wild-type, fresh and dark red flower shoots of the chimera. The dark red flower shoots of the chimera were conserved and cultivated at a large-scale through micropropagation. MS supplemented with 2.0mg/L BA and 0.3mg/L NAA was the optimal medium in which the maximum proliferation ratio (5.2-fold) and rooting rate (88%) were achieved after 6 weeks. Our findings provide an important method to improve the ornamental quality of S. splendens.