Micropropagation of coconut (Cocos nucifera Linn var. pandan) through somatic embryogenesis technique

• Published in: Food Research (Online) Vol. 8; no. Supplementary 7; pp. 28 - 35
• Main Authors: Maslini, J.A., Intan, N.H.A.H.
• Format: Journal Article
• Language: English
• Published: 09.12.2024
• ISSN: 2550-2166; 2550-2166
• DOI: 10.26656/fr.2017.8(S7).5

Coconut, also known as Cocos nucifera, is one of the most recalcitrant species for in vitro regeneration. Conventional coconut breeding has a long lifetime and high heterozygosity, making it a time-consuming, intricate, and costly technique for plant growth. As a result, it is critical to replant most of the world's agricultural land and to develop new soil. This massive undertaking will necessitate planting at least a billion coconut palm trees, which cannot be propagated by seed. Somatic embryogenesis, as a biotechnological alternative, is thus required. Work on this subject has been done in laboratories in numerous countries, with different techniques and explant shapes being investigated. The zygotic embryos are more effective in initiating the callus induction fortified on C1 and C2 formulations added with activated charcoal, gelling agent, and hormone 2, 4- dichlorophenoxyacetic acid (2,4- D). The study has observed that the C1 media successfully initiated callus formation, but the C2 media allowed embryogenic calluses and somatic embryos to develop. From this study, the seedling and regenerated callus were growing well on C3 media. Mature coconut plantlets were acclimated using a combination of sphagnum, sand, and soil mix. The plantlets, on the other hand, could not adapt to their circumstances.