The Effect of Packing on Water Quality Parameters, Survival and NNV Load of Epinephelus coioides Fry after Simulated Transport

• Published in: Journal of acquaculture research & development Vol. 5; no. 6; p. 1
• Main Author: Lee CF, Cheng AC
• Format: Journal Article
• Language: English
• Published: 01.01.2014
• Subjects: Epinephelus coioides; Life Sciences; Marine; Epinephelus coioides; Water quality parameter; NNV load; Grouper; Zeolite; Survival; Simulated transport
• ISSN: 2155-9546; 2155-9546
• DOI: 10.4172/2155-9546.1000264

Water quality parameters, survival rates, and nervous necrosis virus (NNV) loadings of Epinephelus coioides grouper fry packed in 50 L bags after 24 h of simulated transport were examined. All fry packed at 300/bag and with water/oxygen ratios of 10 L/40 L, 12.5 L/37.5 L, and 15 L/35 L at 20 degree C survived with NNV loads of 4.1~7.910 super( 4) at 7 d post-release in seawater. All fish packed at 300/bag and a water/oxygen ratio of 12.5 L/37.5 L at 20 degree C survived with an NNV load of 4.110 super( 3), but fish packed at 25 degree C and 30 degree C survived 100% and 67.4%, and had NNV loads of 1.610 super( 5) and 1.810 super( 6), respectively at 7 d post-release. All fish packed at 200, 300, and 400/bag with a water/oxygen ratio of 12.5 L/37.5 L at 20 degree C survived with DO >5.0 mg/L, CO sub( 2) <95 mg/L, pH >5.8, and ammonia-N <11 mg/L, after 24 h. All fish packed at 200 and 300/bag survived with an NNV load of 1.710 super( 3), whereas fish packed at 400 and 500/bag survived only 60.8% and 42.6% with NNV loads of 2.410 super( 7) and 3.710 super( 7), respectively at 7 d post-release. We concluded that grouper fry packed at 300/bag and a water/oxygen ratio of 12.5 L/37.5 L at 20 and 25 degree C were optimal for maintaining high DO (>5.8 mg/L) and pH (>5.6) and low CO sub( 2) (<89 mg/L), ammonia-N (<10.2 mg/L), and NNV loads (<4.910 super( 3)) after 24 h of simulated transport. The addition of zeolite increased DO and pH while lowering carbon dioxide and ammonia-N as well as lower NNV loads (2.310 super( 3)) during simulated transport.