Correlation between Chemokine CXCL-12 and ist Receptor CXCR4 Expression is Associated with Clinical Prognosis of Gastric Cancer

• Published in: Clinical laboratory (Heidelberg) Vol. 66; no. 4
• Main Authors: Xu, Gang, Lu, Ke, Shen, Minghai, Zhang, Qinghua, Pan, Wanneng, Tang, Zhongzhu
• Format: Journal Article
• Language: English
• Published: Germany 01.04.2020
• Subjects: Chemokine CXCL12 - genetics; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Humans; Kaplan-Meier Estimate; Neoplasm Staging; Prognosis; Receptors, CXCR4 - genetics; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology
• ISSN: 1433-6510
• DOI: 10.7754/Clin.Lab.2019.190217

To analyze the differences in gene expression levels of chemokine CXCL-12 and its receptor CXCR4 in gastric cancer and the relationship between their correlations with the clinical prognosis of gastric cancer. The information on gastric cancer in the TCGA (The Cancer Genome Atlas) database was downloaded from the Broad GDAC FIREHOSE, including CXCL-12 and CXCR4 gene expression data of 415 gastric cancer tissues and 35 normal gastric tissues; clinical information of 392 gastric cancer cases. All patients were divided into either a correlated (significantly higher or lower correlation between CXCL12 and CXCR4 expression) or uncorrelated groups. Wilcoxon rank sum test was used to analyze the differential gene expressions of CXCL-12 and CXCR4 between gastric cancer tissues and normal gastric tissues. Furthermore, one-way analysis of variance and Kaplan-Meier survival analysis were used to analyze the differential gene expressions of CXCL-12 and CXCR4 and the prognosis in patients with different stages of gastric cancer. Gastric cancer patients were divided into two groups according to whether CXCL-12 and CXCR4 gene expressions were correlated or not. Kaplan-Meier survival analysis was used to analyze the three-year survival of the two groups. There were differences between CXCL-12 and CXCR4 expression in 415 gastric cancer tissues and 35 normal gastric tissues. No statistically significant difference between CXCL-12 and CXCR4 was detected in different stages of gastric cancer. There were differences of the five-year survival in different stages of gastric cancer. Further analysis showed that the three-year survival in the correlated group was superior compared to the uncor-related one. The gene expression of CXCL-12 and CXCR4 was significantly different between gastric cancer tissues and normal gastric tissues. Moreover, the correlation between CXCL-12 and CXCR4 gene expression may be used as a predictor of clinical prognosis in patients with gastric cancer.