Group II phospholipase A 2 activates mitogen-activated protein kinase in cultured rat mesangial cells

Group II phospholipase A 2 (PLA 2) is a mediator of inflammation in various disease including glomerulonephritis. We recently found that urinary excretion of PLA 2 was increased in patients with mesangial proliferative glomerulonephritis and that interleukin-1 (IL-1) enhanced platelet derived growth...

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Bibliographic Details
Published inFEBS letters Vol. 370; no. 1; pp. 141 - 145
Main Authors Sugiura, Toshihiro, Wada, Akira, Itoh, Takahito, Tojo, Hiromasa, Okamoto, Mitsuhiro, Imai, Enyu, Kamada, Takenobu, Ueda, Naohiko
Format Journal Article
LanguageEnglish
Published Elsevier B.V 14.08.1995
Subjects
Glomerulonephritis
Group II phospholipase A 2
MAP kinase
Mesangial cell
Glomerulonephritis
BSA
Mesangial cell
IL-1
PDGF
MAP kinase
MBP
LPA
PLA 2
Group II phospholipase A 2
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Summary:Group II phospholipase A 2 (PLA 2) is a mediator of inflammation in various disease including glomerulonephritis. We recently found that urinary excretion of PLA 2 was increased in patients with mesangial proliferative glomerulonephritis and that interleukin-1 (IL-1) enhanced platelet derived growth factor-stimulated mesangial cell proliferation through the action of group II PLA 2 secreted in response to IL-1 stimuli. Here we report signal transducing mechanism through group II PLA 2 in mesangial cells. Group II PLA 2 (1–15 U/ml) rapidly activated mitogen-activated protein (MAP) kinase. IL-1β activated MAP kinase in two phases and the slow activation in the late phase, proceeding in parallel with increased group II PLA 2 secretion elicited by IL-1 treatment, was inhibited by the specific antibody raised against group II PLA 2. This suggests that the late phase activation of IL-1-induced MAP kinase was mediated, at least in part, by secreted group II PLA 2.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00810-V