Enantioselective separation in capillary electrophoresis using a novel mono-6A-propylammonium-β-cyclodextrin as chiral selector

• Published in: Analytica chimica acta Vol. 555; no. 1; pp. 63 - 67
• Main Authors: WEIHUA TANG, MUDERAWAN, I. Wayan, NG, Siu-Choon, SZE ON CHAN, Hardy
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier 05.01.2006
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Other chromatographic methods; Peak resolution; Separation; Enantioselectivity; Capillary electrophoresis; Cationic β-cyclodextrin; Routine analysis; Isomer; Chiral selector; Cyclodextrin; Carboxylic acid; Reproducibility; Chlorides; Enantiomer; Linearity; Enantionmeric separation; 6A-Propylammonium-6A-deoxy-β-cyclodextrin chloride
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2005.08.064

The chiral resolving ability of a novel single-isomer cationic beta-cyclodextrin (CD), mono-6A-propylammonium-6A-deoxy-beta-cyclodextrin chloride (PrAMCD), as a chiral selector in capillary electrophoresis (CE) is reported in this work for the enantioseparation of hydroxy, carboxylic acids and amphoteric analytes. The effect of chiral selector concentration on the resolution was studied. Good resolutions were achieved for hydroxy acids. Optimum resolutions were obtained even at 3.5mM CD concentration for carboxylic acids. The electrophoretic method showed good linearity and reproducibility in terms of migration times and peak areas, which should make it suitable for routine analysis. In addition, baseline chiral separation of a six-acid mixture was achieved within 20min. PrAMCD proved to be an effective chiral selector for acidic analytes.