Mechanism of histamine-induced calcium efflux from cultured bovine adrenal chromaffin cells: possible involvement of an [formula omitted] exchange mechanism

• Published in: Neuroscience letters Vol. 180; no. 2; pp. 281 - 284
• Main Authors: Houchi, Hitoshi, Kitamura, Katsuji, Minakuchi, Kazuo, Ishimura, Yasuko, Okuno, Masaaki, Ohuchi, Takeshi, Oka, Motoo
• Format: Journal Article
• Language: English
• Published: Elsevier Ireland Ltd 01.10.1994
• Subjects: [formula omitted] exchange; Ca 2+ efflux; Chromaffin cell; Histamine; Histamine; Na + Ca 2+ exchange; Chromaffin cell; Ca 2+ efflux
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(94)90539-8

The effect of stimulation of the histamine receptor on Ca 2+ mobilization in cultured bovine adrenal chromaffin cells was examined. Histamine (10 −5 M) increased the intracellular free Ca 2+ ([Ca 2+] i) to a peak in the presence or absence of extracellular Ca 2+, followed by decrease with time. Histamine (10 −8–10 −5 M) also stimulated 45Ca 2+ efflux from cultured bovine adrenal chromaffin cells in a concentration dependent manner. Its stimulatory effect on 45Ca 2+ efflux was inhibited by the specific histamine H 1 receptor antagonist mepyramine. The increase in histamine-stimulated 45Ca 2+ efflux was inhibited by deprivation of extracellular Na + and by the Na + Ca 2+ exchange inhibitor amiloride. In addition, histamine stimulated 22Na + influx into the cells, and this action was inhibited by amiloride. These results suggest that stimulation of the histamine H 1 receptor regulates Na + Ca 2+ exchange in cultured bovine adrenal chromaffin cells.