Validation of alternative capillary electrophoresis detection of STRs using POP-6 polymer and a 22 cm array on a 3130 xl genetic analyzer

Highlights • A detection method is presented that utilizes POP-6 polymer and 22 cm array. • Detection time is reduced by ∼40% and sample throughput is increased ∼67%. • Full allele concordance is achieved without panel and bin modifications. • Resolution is slightly improved for smaller sized allele...

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Bibliographic Details
Published inForensic science international : genetics Vol. 22; pp. 113 - 127
Main Authors Connon, Catherine C, LeFebvre, Aaron K, Benjamin, Robert C
Format Journal Article
LanguageEnglish
Published 01.05.2016
Subjects
Pathology
Forensic science
Capillary electrophoresis
Precision
Alternative detection
Resolution
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Summary:Highlights • A detection method is presented that utilizes POP-6 polymer and 22 cm array. • Detection time is reduced by ∼40% and sample throughput is increased ∼67%. • Full allele concordance is achieved without panel and bin modifications. • Resolution is slightly improved for smaller sized alleles (<200 b). • One base resolution is achievable for allele sizes of up to 300 b.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2016.02.006