Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An In Vitro Study

• Published in: The journal of contemporary dental practice Vol. 25; no. 4; pp. 313 - 319
• Main Authors: Alshawkani, Hamed A, Mansy, Mohamed, Ankily, Mahmoud Al, Shamel, Mohamed
• Format: Journal Article
• Language: English
• Published: India 01.04.2024
• Subjects: Alkaline Phosphatase - metabolism; Bone Morphogenetic Protein 2; Cell Differentiation - drug effects; Cell Survival - drug effects; Cells, Cultured; Ceramics; Core Binding Factor Alpha 1 Subunit; Dental Pulp - cytology; Extracellular Matrix Proteins; Humans; In Vitro Techniques; Low-Level Light Therapy - methods; Odontogenesis - drug effects; Phosphoproteins; Regeneration - drug effects; Root Canal Filling Materials - pharmacology; Sialoglycoproteins; Stem Cells - drug effects; Odontogenic genes; Photobiomodulation Vital pulp therapy; Dental pulp stem cells; Bioceramic
• ISSN: 1526-3711; 1526-3711
• DOI: 10.5005/jp-journals-10024-3676

This study aims to assess the synergistic effect of utilizing a bioceramic sealer, NeoPutty, with photobiomodulation (PBM) on dental pulp stem cells (DPSCs) for odontogenesis. Dental pulp stem cells were collected from 10 premolars extracted from healthy individuals. Dental pulp stem cells were characterized using an inverted-phase microscope to detect cell shape and flow cytometry to detect stem cell-specific surface antigens. Three experimental groups were examined: the NP group, the PBM group, and the combined NP and PBM group. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) experiment was conducted to assess the viability of DPSCs. The odontogenic differentiation potential was analyzed using Alizarin red staining, RT-qPCR analysis of odontogenic genes DMP-1, DSPP, and alkaline phosphatase (ALP), and western blot analysis for detecting BMP-2 and RUNX-2 protein expression. An analysis of variance (ANOVA) followed by a -test was employed to examine and compare the mean values of the results. The study showed a notable rise in cell viability when NP and PBM were used together. Odontogenic gene expression and the protein expression of BMP-2 and RUNX-2 were notably increased in the combined group. The combined effect of NeoPutty and PBM was significant in enhancing the odontogenic differentiation capability of DPSCs. The synergistic effect of NeoPutty and PBM produced the most positive effect on the cytocompatibility and odontogenic differentiation potential of DPSCs. Creating innovative regenerative treatments to efficiently and durably repair injured dental tissues. How to cite this article: Alshawkani HA, Mansy M, Al Ankily M, . Regenerative Potential of Dental Pulp Stem Cells in Response to a Bioceramic Dental Sealer and Photobiomodulation: An Study. J Contemp Dent Pract 2024;25(4):313-319.