Differences de specificite entre les methylases de myelome et de foie vis-a-vis du tRNA d' E. coli K 12
This paper describes an attempt to find a difference between the patterns of methylation of E. coli tRNA by extracts of two mouse tissues. Two samples of tRNAs, methylated in two separate experiments with extracts of myeloma and of liver in presence of either 14C or 3H S-Adenosyl-L-Methionine, were...
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| Published in | FEBS letters Vol. 4; no. 4; pp. 347 - 350 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | French English |
| Published |
Elsevier B.V
01.08.1969
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| Online Access | Get full text |
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| Summary: | This paper describes an attempt to find a difference between the patterns of methylation of
E. coli tRNA by extracts of two mouse tissues. Two samples of tRNAs, methylated in two separate experiments with extracts of myeloma and of liver in presence of either
14C or
3H S-Adenosyl-L-Methionine, were pooled and fractionated together on a RPC column. The results show a difference in the specificities of the two extracts. Chromatography on DEAE Sephadex suggests that the tRNA Met is methylated by the enzymes on the myeloma, while enzymes from liver react very little, if at all, with that particular tRNA species. Studies have been undertaken in order to find out whether similar differences can also be demonstrated in homologous systems. |
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| ISSN: | 0014-5793 1873-3468 |
| DOI: | 10.1016/0014-5793(69)80273-5 |