Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage

Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific f...

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Bibliographic Details
Published inSigmulbyeong yeon'gu Vol. 20; no. 1; pp. 21 - 24
Main Authors Choi, Jin Su, Yang, Seul Gi, Song, Jeong Young, Kim, Hong Gi
Format Journal Article
LanguageEnglish
Published Hanrimwon Publishing Company 31.03.2014
Subjects
Clubroot pathogen
Detection
Plasmodiophora brassicae
Quantitative real-time PCR
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Summary:Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae.
ISSN:1598-2262
2233-9191
DOI:10.5423/RPD.2014.20.1.021