Determination of the cannabinoid receptors in mouse × rat hybridoma NG108-15 cells and rat GH4C 1 cells

The cannabinoid receptors expressed in the mouse neuroblastoma × rat glioma NG108-15 cell and the rat pituitary tumor GH4C1 cell were determined by polymerase chain reaction, dideoxysequencing and pharmacologically. The CBI but not the C132 or CB1A cannabinoid receptor was found in both cell lines....

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Published inNeuroscience letters Vol. 212; no. 2; pp. 123 - 126
Main Authors Ho, Begonia Y., Zhao, Jinchuan
Format Journal Article
LanguageEnglish
Published Elsevier Ireland Ltd 01.07.1996
Subjects
Cannabinoid CB1 receptor
G protein-coupled receptor
GH4C1 cells
Marijuana
NG108-15 cells
G protein-coupled receptor
Marijuana
GH4C1 cells
NG108-15 cells
Cannabinoid CB1 receptor
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Summary:The cannabinoid receptors expressed in the mouse neuroblastoma × rat glioma NG108-15 cell and the rat pituitary tumor GH4C1 cell were determined by polymerase chain reaction, dideoxysequencing and pharmacologically. The CBI but not the C132 or CB1A cannabinoid receptor was found in both cell lines. The cDNA identified in GH4C1 cells corresponds to the rat CB 1 receptor. Interestingly, NG108-15 cells express two distinct cDNAs, one corresponds to the rat and the other to the mouse CB1 receptor. The newly developed CB1 receptor selective antagonist SR141716A was found to reverse cannabinoid agonist (WIN55212-2 or CP55940)-induced adenylyl cyclase inhibition. These results provide more direct evidence that the CB 1 receptor is mediating the pharmacological actions of cannabinoids in NG108-15 and GH4C1 cells.
ISSN:0304-3940
1872-7972
DOI:10.1016/0304-3940(96)12792-0