The relationship between point mutation and abnormal expression of c-fms oncogene in hepatocellular carcinoma

• Published in: Hepatobiliary & pancreatic diseases international Vol. 3; no. 1; pp. 86 - 89
• Main Authors: Yang, Dong-Hua, Huang, Wei, Cui, Jun, Shu, Jian-Chang, Tang, Shao-Hui, Zhang, Wen-Jie, Liang, Jie-Hua
• Format: Journal Article
• Language: English
• Published: Singapore 01.02.2004
• Subjects: Adult; Aged; Base Sequence; Blotting, Northern; Blotting, Western; c-fms; carcinoma; Carcinoma, Hepatocellular - genetics; Case-Control Studies; Chi-Square Distribution; expression; Female; Gene Amplification; Gene Expression Regulation, Neoplastic; Genes, fms - genetics; hepatocellular; Humans; Liver Neoplasms - genetics; Male; Middle Aged; Molecular Sequence Data; mutation; oncogene; Point Mutation; Polymerase Chain Reaction; Proto-Oncogenes - genetics; Reference Values; RNA, Messenger - analysis; Sensitivity and Specificity
• ISSN: 1499-3872

Recent research found abnormal expression of the c-fms oncogene, which encodes the macrophage colony-stimulating factor receptor (CSF-1R), in several human carcinomas including hepatocellular carcinoma (HCC). But the relationship between the point mutation and abnormal expressing of c-fms oncogene in HCC was not clear. This study is to investigate the relationship between point mutation and abnormal expression of c-fms oncogene in hepatocellular carcinoma (HCC) and to clarify the mechanism of HCC. The expression of c-fms oncogene at different levels of cell, protein and transcription was observed using immune histological ABC, Western blot and Northern blot. PCR-single strand conformation polymorphism and gene sequencing were used to detect the mutation of c-fms in HCC tissues and their surrounding tissues of 30 patients. The expression of c-fms was significantly higher in HCC tissues than in their surrounding tissues (P<0.01). Point mutation of Leu (TTG)-->Ser (TCG) at codon 301 of c-fms amino acids was observed in 21.4% (3/14) HCC tissues. No mutation of c-fms oncogene was detected in the surrounding cancerous tissues. Point mutation at codon 301 of c-fms oncogene is one of the mechanisms of abnormal over-expression in HCC.