A scaling relationship between thermodynamic and hydrodynamic interactions in protein solutions
Weak protein interactions are associated with a broad array of biological functions and are often implicated in molecular dysfunction accompanying human disease. In addition, these interactions are a critical determinant in the effective manufacturing, stability, and administration of biotherapeutic...
Saved in:
Published in | Biophysical journal |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.10.2024
|
Online Access | Get full text |
Cover
Loading…
Summary: | Weak protein interactions are associated with a broad array of biological functions and are often implicated in molecular dysfunction accompanying human disease. In addition, these interactions are a critical determinant in the effective manufacturing, stability, and administration of biotherapeutic proteins. Despite their prominence, much remains unknown about how molecular attributes influence the hydrodynamic and thermodynamic contributions to the overall interaction mechanism. To systematically probe these contributions, we have evaluated self-interaction in a diverse set of proteins that demonstrate a broad range of behaviors from attractive to repulsive. Analysis of the composite trending in the data provides a convenient interconversion among interaction parameters measured from the concentration dependence of the molecular weight, diffusion coefficient, and sedimentation coefficient, as well as insight into the relationship between thermodynamic and hydrodynamic interactions. We find relatively good agreement between our data and a model for interacting hard spheres in the range of weak self-association. Additionally, we propose an empirically-derived, general scaling relationship applicable across a broad range of self-association and repulsive behaviors. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3495 1542-0086 1542-0086 |
DOI: | 10.1016/j.bpj.2024.09.032 |