Measurement of NADPH Oxidase Activity in Plants

NADPH oxidase is a membrane-bound enzyme that generates (O2-) by transferring electrons from NADPH to molecular oxygen O2. O2-is spontaneously dismasted to the more stable form H2O2. Both O2-and H2O2 are forms ofreactive oxygen species (ROS), which are involved in regulation of many cellular activit...

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Bibliographic Details
Published inBio-protocol Vol. 2; no. 20
Main Authors Amita Kaundal, Clemencia Rojas, Kirankumar Mysore
Format Journal Article
LanguageEnglish
Published Bio-protocol LLC 01.10.2012
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Summary:NADPH oxidase is a membrane-bound enzyme that generates (O2-) by transferring electrons from NADPH to molecular oxygen O2. O2-is spontaneously dismasted to the more stable form H2O2. Both O2-and H2O2 are forms ofreactive oxygen species (ROS), which are involved in regulation of many cellular activities such as transcription, intracellular signaling, and host defense. The NADPH oxidase - dependent generation of O2- in total membrane fraction of plant tissue has been determined by the reduction of the tetrazolium salt XTT by O2-. In the presence of O2-, XTT generates a soluble yellow formazan that can be quantified spectrophotometrically.
ISSN:2331-8325
DOI:10.21769/BioProtoc.278