Separation and expansion of tumor infiltrating lymphocytes of digestive system in vitro and their cytotoxicity

• Published in: Journal of Tongji Medical University Vol. 15; no. 4; p. 220
• Main Authors: Chen, D D, Tan, Q F, Tao, K X
• Format: Journal Article
• Language: English
• Published: China 1995
• Subjects: Adult; Aged; Cytotoxicity, Immunologic; Female; Humans; Immunotherapy, Adoptive; Interleukin-2 - immunology; Liver Neoplasms - immunology; Liver Neoplasms - pathology; Lymphocytes, Tumor-Infiltrating - cytology; Lymphocytes, Tumor-Infiltrating - immunology; Male; Middle Aged; Recombinant Proteins - immunology; Rectal Neoplasms - immunology; Rectal Neoplasms - pathology; Stomach Neoplasms - immunology; Stomach Neoplasms - pathology
• ISSN: 0257-716X
• DOI: 10.1007/BF02887949

Primary tumor tissues in the digestive system were harvested from 15 patients. By mincing, enzymatic digestion and gradient density separation, sufficient TILs (> x 10(6)) were obtained from 13 of 15 (88.7%) patients in vitro in the presence of 500 mu/ml of recombinant interleukin-2 and 5% fetal calf serum after one month culture. 92.3% (12/13) of TILs proliferated well in vitro (92.3%). TILs expanded from 10(/)-folds to 10(3)-folds after being cultured for one month. CD25+ cell of the most fresh TILs was more than that of peripheral blood lymphocytes. CD25+ cells of TILs during 4th week of the culture was significantly greater (P < 0.01) than that of fresh TILs. CD4+/CD8+ ratio was decreased during four culture weeks because of increase of CD8 cells. By using modified colorimetric MTT assay for measuring activity of TILs against various tumor cells the results showed that cytotoxicity of gastro-intestinal TILs in autologous tumor cells is greater than on the other tumor cells.