Citrate synthase from the thermophilic archaebacterium Thermoplasma acidophilum

The gene encoding the citric acid cycle enzyme, citrate synthase, has been cloned from the thermoacidophilic archaebacterium, Thermoplasma acidophilum. We report the sequencing of this gene and its flanking regions, and the derived amino acid sequence of the enzyme is compared by multiple‐sequence a...

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Bibliographic Details
Published inEuropean journal of biochemistry Vol. 194; no. 3; pp. 839 - 844
Main Authors SUTHERLAND, Katharine J., HENNEKE, Christina M., TOWNER, Paul, HOUGH, David W., DANSON, Michael J.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.12.1990
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Summary:The gene encoding the citric acid cycle enzyme, citrate synthase, has been cloned from the thermoacidophilic archaebacterium, Thermoplasma acidophilum. We report the sequencing of this gene and its flanking regions, and the derived amino acid sequence of the enzyme is compared by multiple‐sequence alignment analysis with those of citrate synthases from eubacterial and eukaryotic organisms. The similarity is < 30% between the archaebacterial and non‐archaebacterial sequences, although the majority of residues implicated in the catalytic action of the enzyme have been conserved across all three kingdoms. The cloned archaebacterial gene has been expressed in Escherichia coli to produce catalytically active citrate synthase. This is the first reported sequence of citrate synthase from the archaebacteria.
Bibliography:The novel nucleotide sequence data published here have been deposited with the EMBL sequence data bank and are available under the accession number X55282.
Note.
ISSN:0014-2956
1432-1033
DOI:10.1111/j.1432-1033.1990.tb19477.x