In vitro Barnardia japonica (Thunb.) Schult. et Schult. fil. micropropagation by direct regeneration

• Published in: Botanica Pacifica : journal of plant science and conservation
• Main Authors: Pianova, Anastasiya S., Salokhin, Aleksey V., Lonchakova, Tat’yana E., Sabutski, Yuri E.
• Format: Journal Article
• Language: English
• Published: 01.11.2021
• ISSN: 2226-4701; 2410-3713
• DOI: 10.17581/bp.2021.10208

In vitro micropropagation of rare Far Eastern species Barnardia japonica by the direct regeneration method was studied for the first time. The effect of different concentrations of 6-benzylaminopurine and kinetin in combination with auxins on the regeneration potential of young seedlings using Murashige and Skoog media was estimated. It was found that the largest number of new plants (61 new shoots on average) is formed when explants are cultivated on the medium with a high concentration of kinetin (10 mg/L) and a minimum of α-naphthylacetic acid (0.1 mg/L). The resulting plants are rooted easily on a hormone-free medium of the same mineral composition. The least effective for the mass production of plants by direct regeneration was a medium with a combination of 2 mg/L kinetin and 1 mg/L α-naphthylacetic acid. The developed approach can be applied for the effective propagation of Barnardia japonica and returning the species to its natural habitat.