Evidence for the Existence of Serotonin Uptake Inhibitor-Like Substances in Human Cerebrospinal Fluid

Addition of human cerebrospinal fluid (CSF) induced a marked inhibition of 3H-paroxetine binding to the monkey cortical membranes, while the specific binding of 3H-imipramine was slightly inhibited. Moreover, 3H-serotonin (5-hydroxytryptamine, 5-HT) uptake inhibition in the monkey cortical synaptoso...

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Bibliographic Details
Published inJapanese journal of pharmacology Vol. 56; no. 3; pp. 297 - 302
Main Authors Goto, Shinichiro, Egashira, Toru, Yamanaka, Yasumitsu
Format Journal Article
LanguageEnglish
Published 1991
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Summary:Addition of human cerebrospinal fluid (CSF) induced a marked inhibition of 3H-paroxetine binding to the monkey cortical membranes, while the specific binding of 3H-imipramine was slightly inhibited. Moreover, 3H-serotonin (5-hydroxytryptamine, 5-HT) uptake inhibition in the monkey cortical synaptosomes was also increased as the volume of added CSF was increased. Scatchard analysis of specific 3H-paroxetine binding with human CSF showed non-competitive kinetics, although CSF was competitive with 3H-imipramine binding. The inhibitory effect of human CSF on 5-HT uptake was non-competitive in nature. The endogenous substances in human CSF most probably act at the recognition site labeled with 3H-paroxetine. Moreover, occupation of this site by the endogenous substances is likely to inhibit the 5-HT uptake process.
ISSN:0021-5198
1347-3506
DOI:10.1016/S0021-5198(19)39856-7