PSX-B-25 Milk productivity and zinc species analysis in cattle depending on the level of lead accumulation

• Published in: Journal of animal science Vol. 99; no. Supplement_3; pp. 272 - 273
• Main Authors: Kazakova, Tatiana, Marshinskaia, Olga, Notova, Svetlana
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 01.11.2021
• Subjects: Accumulation; Albumins; Animals; Blood; Cattle; Citrates; Dimers; Globulins; High performance liquid chromatography; Inductively coupled plasma mass spectrometry; Lead; Ligands; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Metabolism; Milk; Productivity; Proteins; Research facilities; Speciation; Species; Statistical analysis; Zinc; speciation analysis; milk productivity; zinc; lead; HPLC-ICP-MS
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skab235.499

Abstract The aim was to study the milk productivity and zinc species in dairy Holstein cow blood serum depending on the level of lead accumulation. Fat and protein content in milk was assessed by the FIL-IDF procedure. The major zinc species in dairy cow blood serum were tested using a hybrid high-performance liquid chromatography-inductively coupled plasma-mass spectrometry technique. The lead content in the blood serum of all animals was in the intervals of the physiological standard. Animals with low-normal values (Pb concentration up to the 25th percentile) and high-normal values (Pb concentration above the 75th percentile) were taken to form comparison groups. Animals with high-normal values of lead had a statistically significant decrease of milk yield by 21.8% and daily production of fat by 16.4%. There was a tendency towards a decrease in the yield of protein and its percentage. It was found that the zinc content in all studied fractions (α 2-macro-globulin tetramer, α 2-macro-globulin dimer, albumin, citrates, total content) was statistically significantly lower (more than 4.8 times) in the group with high-normal values of lead. The change in the redistribution of zinc-binding ligands was found depending on the level of lead in the blood. The relative content of the α2-macro-globulin tetramer and α2-macro-globulin dimer was significantly higher by 7% and by 13% in the group with high-normal values of lead. There was the decrease in the level of animal productivity, the metal-ligand forms of zinc underwent changes despite the absence of a significant difference in the total level of lead in the blood serum. This indicates a high potential for the use of speciation analysis in the laboratory diagnosis and allows predicting and correcting zinc metabolism. The studies were performed in accordance with the research plan for 2019–2021 of the Federal Research Center of Biological Systems and Agrotechnologies RAS No. 0526-2019-0001.