Novel Technology for Protein-Protein Interaction-based Targeted Drug Discovery

We have developed a simple but highly efficient in-cell protein-protein interaction (PPI) discovery system based on the translocation properties of protein kinase C- and its C1a domain in live cells. This system allows the visual detection of trimeric and dimeric protein interactions including cytos...

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Bibliographic Details
Published inJournal of analytical science and technology Vol. 2; no. Supplement A; pp. A61 - A65
Main Authors Hwang, Jung Me, Lee, Kyung-Bok, Choi, Jong-Soon, Rho, Jaerang, Kim, Gun-Hwa, Kim, Seung Il, Kim, Soohyun, Lee, Zee-Won
Format Journal Article
LanguageEnglish
Published SpringerOpen 22.12.2011
Subjects
Interaction
Protein
Translocation
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Summary:We have developed a simple but highly efficient in-cell protein-protein interaction (PPI) discovery system based on the translocation properties of protein kinase C- and its C1a domain in live cells. This system allows the visual detection of trimeric and dimeric protein interactions including cytosolic, nuclear, and/or membrane proteins with their cognate ligands. In addition, this system can be used to identify pharmacological small compounds that inhibit specific PPIs. These properties make this PPI system an attractive tool for screening drug candidates and mapping the protein interactome.
ISSN:2093-3134
2093-3371
DOI:10.5355/JAST.2011.A61