Immunohistochemical expression of p16INK4a in premalignant lesions and malignant tumours of cervix

Background: Cervical carcinoma is the third most common cancer in women worldwide and firth most common cause of cancer related deaths. Human Papilloma virus, the most common cause of cervical cancer, causes functional inactivation of pRb, resulting in overexpression of p16INK4a. The overexpression...

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Bibliographic Details
Published inInternational Journal of Reproduction, Contraception, Obstetrics and Gynecology Vol. 12; no. 6; pp. 1813 - 1819
Main Authors Sandhu, Parminder J.S, Nibhoria, Sarita, Sandhu, Vaneet K, Kaur, Harpreet
Format Journal Article
LanguageEnglish
Published International Journal of Reproduction, Contraception, Obstetrics and Gynecology 01.06.2023
Subjects
Cervical cancer
Cervix dysplasia
Diagnosis
Gene expression
Genetic aspects
Health aspects
Immunohistochemistry
Methods
Tumor suppressor genes
India
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Summary:Background: Cervical carcinoma is the third most common cancer in women worldwide and firth most common cause of cancer related deaths. Human Papilloma virus, the most common cause of cervical cancer, causes functional inactivation of pRb, resulting in overexpression of p16INK4a. The overexpression of p16INK4a correlates well with the degree of cervical dysplasia and neoplasia. The present study is done to determine the IHC expression of p16INK4a so that it may be used as a biomarker for HPV and may play a role in the prevention, diagnosis and prognosis of cervical neoplasm. Methods: The present study was conducted on 60 formalin-fixed, paraffin-embedded specimens of cervical neoplasms, which comprised 47 cases of squamous cell carcinoma followed by 8 cases of adenocarcinoma, 3 cases of HSIL and 2 cases of LSIL. These were then subjected to IHC by p16INK4a. Cytoplasmic and nuclear positivity for p16INK4a was noted. Results: Overall 95% cases were positive for p16INK4a expression, 100% cases of adenocarcinoma, 98% cases of squamous cell carcinoma, 100% cases of HSIL and 0% cases of LSIL were positive for p16 expression. Conclusions: The present study showed statistical correlation of immunoreactivity of p16INK4a with histological type was clinically significant (p value <0.0001). In our study, p16INK4a immunohistochemical expression increased with increasing degree of dysplasia and with presence of cervical carcinoma. Keywords: p16INK4a, Cervical carcinoma, IHC, CIN, HSIL, LSIL
ISSN:2320-1770
2320-1789
DOI:10.18203/2320-1770.ijrcog20231561