Estimation of Biological Toxicity by Copper oxychloride on Pisum sativum L. and Vigna radiata L

• Published in: Indian journal of agricultural research no. Of
• Main Authors: Paul, Anirban, Bairagya, Koyel Das, Banerjee, Nirmalya, Roy, Anjalika
• Format: Journal Article
• Language: English
• Published: 01.02.2022
• ISSN: 0367-8245; 0976-058X
• DOI: 10.18805/IJARe.A-5606

Background: This research work describes about toxicity estimation of commonly used fungicide copper oxychloride using Pisum sativum L. and Vigna radiata L. as a bioindicator. Methods: The seeds of P. sativum and V. radiata were treated with different concentrations of fungicide. Biological toxicity measured by seed germination percentage, R/P ratio, total leaf chlorophyll, total leaf proline, total seed protein, mitotic index, abnormality index and mitotic inhibition. Result: Linear regression analysis showed that seed germination percentage, total seed protein, abnormality index and mitotic inhibition show positive correlation with increasing concentration of fungicide copper oxychloride. The R/P ratio of 4th and 8th days, mitotic index, total leaf chlorophyll and leaf proline have negative correlation along increasing concentration of fungicide. However total leaf proline of V. radiata at 2% concentration of fungicide is abruptly higher than control and other concentrations. The application of copper oxychloride at lower concentration can be used as a safe fungicide.