In vitro cytotoxicity and ex-vivo genotoxicity in compounds from Waltheria viscosissima A. St. Hil. Brazilian species of the Malvaceae family sensu lato

• Published in: Revista colombiana de ciencias químico-farmacéuticas Vol. 51; no. 3
• Main Authors: Pereira de Sousa, Aleson, Leite Ferreira, Maria Denise, Cordeiro, Laísa Vilar, Souza, Maria de Fátima Vanderlei de, Souza, Helivaldo Diógenes da Silva, Silveira e Sá, Rita de Cássia da, Oliveira Filho, Abrahão Alves de
• Format: Journal Article
• Language: English
• Published: Bogota Universidad Nacional de Colombia 2022
• Subjects: Composting; Cytotoxicity; Genotoxicity; Hemolysis; Herbal medicine; In vitro methods and tests; Natural products; Toxicity
• ISSN: 0034-7418; 1909-6356
• DOI: 10.15446/rcciquifa.v51n3.100263

Introduction: Alternative therapies using medicinal plants and herbal medicines have been growing worldwide, as natural treatments have low cost and less toxic effect compared to conventional drugs available. Aim: To evaluate in vitro cytotoxicity and ex-vivo genotoxicity of Brazilian species of the Malvaceae family: Waltheria viscosissima A. St. Hil. In vitro methods were used to verify the cytotoxic potential through hemolytic and antihemolytic assays and the ex-vivo genotoxic analysis. Results: The Crude Etanolic Extract (CEE) and Cloroformic Fraction (CF) was obtained in vegetal sample used on this study; the CEE-Wv and CF-Wv products exhibited the lowest cytotoxic potential with hemolysis less than 40% in all concentrations evaluated. A moderate anti-hemolytic effect was described in all samples treated with 500 and 1000 μg/mL, with hemolysis <60 %. In addition, the compounds showed low ex-vivo genotoxic effect with a general index of normal cells greater than 84 % at all concentrations. Conclusion: The results suggest a low toxic profile of the compounds obtained from species of the Malvaceae family, indicating safe limits for the use of these natural products.