Corilagin: First Time Isolation from the Whole Plant of Phyllanthus maderaspatensis L

• Published in: Pharmacognosy communications Vol. 9; no. 4; pp. 135 - 138
• Main Authors: Kochumadhavan, Abhilash, Mangal, Priyanka, Kumar, LM Sharath, Meenakshi, Badugu Madhura, Venkanna, Babu Uddagiri, Muguli, Ganesh
• Format: Journal Article
• Language: English
• Published: Bangalore EManuscript Services 01.10.2019
• Subjects: Chemicals; Chromatography; Column chromatography; Extraction; Glucose; High performance liquid chromatography; Liquid chromatography; Methods; Molecular structure; Natural products; NMR; Nuclear magnetic resonance; Organic chemistry; Phyllanthus; Phytochemicals; Product development; Quality control; R&D; Research & development; Retention time; Silica; Silicon dioxide; Standardization; Mumbai India; India
• ISSN: 2249-0167; 2249-0159; 2249-0159; 2249-0167
• DOI: 10.5530/pc.2019.4.28

Introduction: Corilagin, an ellagitannin (chemical name ß-1-0-galloyl-3,6-(R)-hexahydroxydiphenoyl-d-glucose), was previously reported from other species of Phyllanthus except Phyllanthus maderaspatensis L. Therefore, the aim of the study was to isolate corilagin from P maderaspatensis and report it as chemo-marker. Methods: The whole plant of P maderaspatensis was subjected to hot extraction and partitioning, followed by repeated column chromatography (silica, Diaion HP-20 and Prep HPLC). The observed LC-MS/MS and NMR data were compared with reported literature to verify the structure of the isolated molecule as corilagin. Results: The LC-MS/MS chromatogram showed a peak at retention time 22.05 min, with m/z 633 for [M - H]- along with characteristic major fragments at m/z 463 [M - 170 - H]- and m/z 301 [M - 332 - H]-. The NMR results were also in accordance with literature. Conclusion: This is the first report on isolation of corilagin from P maderaspatensis whole plant for standardization and quality control of the plant.