DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam

• Published in: Vietnam Journal of Science, Technology and Engineering Vol. 60; no. 2; pp. 56 - 64
• Main Authors: Bui, Manh Minh, Vu, Anh Tuan, Vu, Phuong Nhung, Pham, Quang Cu, Nguyen, Dang Ton, Huynh, Thi Thu Hue
• Format: Journal Article
• Language: English
• Published: Vietnam Ministry of Science and Technology 30.06.2018
• Subjects: Bayesian inference; DNA barcodes; ITS; maximum likelihood; medicinal plants; phylogenetic
• ISSN: 2525-2461; 2615-9937
• DOI: 10.31276/VJSTE.60(2).56

Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives.DNA barcodes promise to be a precise and reliable tool for distinguishing the processed Huyen-sam materials from their counterfeits. However, studies about using DNA barcodes for classification of Scrophularia L. in Vietnam are not available. Here, we conducted a taxonomic analysis of eight Scrophularia L. samples collected from the mountain areas of Northern Vietnam. Based on the combined sequence data of ribosomal nuclear ITS, a part of chloroplast rbcL gene and trnL-trnF intergenic spacer, phylograms of Scrophularia L.were generated by both Bayesian inference and maximum likelihood bootstrap method. The phylogenetic analysis showed that the tested samples have a sister relationship to S. ningpoensis. Hopefully, the analysis strategy that we used would contribute to further phylogenetic analyses of medicinal plants of Vietnam in the future.