DNA barcoding, an approach for molecular identification of Huyen-sam (Scrophularia L.) samples collected in Northern Vietnam
Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives.DNA barcodes promise to be a p...
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Published in | Vietnam Journal of Science, Technology and Engineering Vol. 60; no. 2; pp. 56 - 64 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Vietnam Ministry of Science and Technology
30.06.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Huyen-sam (Vietnamese name) which belongs to Scrophularia L. genus is a valuable herb. This medicinal plant is classified as Scrophularia ningpoensis Hemsl. Huyen-sam roots, which contain a large amount of bioactive compounds, have a similar morphology to its relatives.DNA barcodes promise to be a precise and reliable tool for distinguishing the processed Huyen-sam materials from their counterfeits. However, studies about using DNA barcodes for classification of Scrophularia L. in Vietnam are not available. Here, we conducted a taxonomic analysis of eight Scrophularia L. samples collected from the mountain areas of Northern Vietnam. Based on the combined sequence data of ribosomal nuclear ITS, a part of chloroplast rbcL gene and trnL-trnF intergenic spacer, phylograms of Scrophularia L.were generated by both Bayesian inference and maximum likelihood bootstrap method. The phylogenetic analysis showed that the tested samples have a sister relationship to S. ningpoensis. Hopefully, the analysis strategy that we used would contribute to further phylogenetic analyses of medicinal plants of Vietnam in the future. |
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ISSN: | 2525-2461 2615-9937 |
DOI: | 10.31276/VJSTE.60(2).56 |