3060 – PDGFRΒ+ CELLS PLAY A DUAL ROLE AS HEMATOPOIETIC PRECURSORS AND NICHE CELLS DURING MOUSE ONTOGENY

• Published in: Experimental hematology Vol. 111; pp. S74 - S75
• Main Authors: Crisan, Mihaela, da bandeira, Diana Sa, Kilpatrick, Alastair, Marques, Madalena, Gomez-salazar, Mario, Ventura, Telma, Gonzalez, Zaniah, Stefancova, Dorota, Rossi, Fiona, Vink, Chris, Beltran, Mariana, Henderson, Neil, Jung, Bongnam, Van Der Linden, Reinier, Werken, Harmen Van De, Van IJcken, Wilfred, Betsholtz, Christer, Forbes, Stuart, Cuervo, Henar
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 2022
• ISSN: 0301-472X; 1873-2399
• DOI: 10.1016/j.exphem.2022.07.116

Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of these clinically important cells in vitro.