Isolation and Characterization of the Eicosapentaenoic Acid Biosynthesis Gene Cluster from Shewanella sp. BR-2

• Published in: Journal of microbiology and biotechnology Vol. 19; no. 9; pp. 881 - 887
• Main Authors: Lee, S.J., Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea, Seo, P.S., Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea, Kim, C.H., Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea, Kwon, O.S., Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea, Hur, B.K., Inha University, Incheon, Republic of Korea, Seo, J.W., Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.09.2009; 한국미생물·생명공학회
• Subjects: ACIDE EICOSAPENTAENOIQUE; ACIDO EICOSAPENTAENOICO; Amino Acid Sequence; Animals; Biological and medical sciences; biosynthesis gene cluster; Biotechnology; EICOSAPENTAENOIC ACID; Eicosapentaenoic Acid - biosynthesis; Escherichia coli - genetics; Fishes - microbiology; Fundamental and applied biological sciences. Psychology; Intestines - microbiology; Molecular Sequence Data; Multigene Family; Organisms, Genetically Modified - genetics; Phylogeny; PKS-like module; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Shewanella - classification; Shewanella - genetics; Shewanella - isolation & purification; Shewanella - metabolism; Shewanella sp. BR-2; 생물학; Gene cluster; Biosynthesis; BR-2; Shewanella; n-3 fatty acid; Characterization; biosynthesis gene cluster; Bacteria; Vibrionaceae; Isolation; Eicosapentaenoic acid; Shewanella sp; PKS-like module
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.0902.0090

Forty-four eicosapentaenoic acid (EPA)-producing microbial strains were isolated from the intestines of marine fishes. Among them, one strain showing a maximum level of EPA (4.78% of total fatty acids) was identified as Shewanella sp. BR-2 on the basis of its 16S rRNA sequence. The EPA content reached a maximum level during the mid-exponential phase of cell growth, and gradually decreased with further growth of the cells. A cosmid DNA including the EPA biosynthesis gene cluster consisting of pfaA-E was isolated from a cosmid library of genomic DNA of Shewanella sp. BR-2, named pCosEPA-BR2. An E. coli clone harboring pCosEPA-BR2 produced EPA at a maximum level of 7.5% of total fatty acids, confirming the EPA biosynthesis activity of the cloned gene cluster.