Influence of cytostatics on relative gene expression in redox status, apoptosis and migration colorectal carcinoma model system

Colorectal cancer is one of the leading causes of mortality worldwide. It is the most common malignancy and there is a need for new approaches in therapies. Surgery, radiation and chemotherapy are the key components of colon cancer treatment. Besides common chemotherapy, alternative therapies are be...

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Published inKragujevac Journal of Science Vol. 2023; no. 45; pp. 159 - 177
Main Authors Pavić, Jelena, Živanović, Marko, Virijević, Katarina, Tanasković, Irena, Stanković, Vesna, Marić, Nebojša, Cvetković, Danijela, Filipović, Nenad
Format Journal Article
LanguageEnglish
Published University of Kragujevac, Faculty of Science 2023
Subjects
cell migration
chemotherapy
colon cancer
gene expression
redox status
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Summary:Colorectal cancer is one of the leading causes of mortality worldwide. It is the most common malignancy and there is a need for new approaches in therapies. Surgery, radiation and chemotherapy are the key components of colon cancer treatment. Besides common chemotherapy, alternative therapies are being studied to increase treatment effectiveness and reduce side effects. In this article, colorectal carcinoma cells were treated with chemotherapeutics and relative gene expression was investigated for the genes coding cytoskeleton proteins: CDH1, CTNNB1 and CDH2; for redox status genes: GPX1, GPX2, GPX3, GPX4, TXNRD1, GSTP1, NFE2L2, NFKB1, HIF1A; and for apoptosis genes: CASP3, CASP8, CASP9, FAS, BCL-2 and BAX. The results of our research showed that some concentrations of chemotherapeutics increased the expression of certain genes. Molecular alterations that lead to colorectal cancer can determine appropriate and effective treatment - chemotherapeutics, as well as the design of direct therapeutic targets.
ISSN:1450-9636
2466-5509
DOI:10.5937/KgJSci2345159P