Studies on platelet-activating factor (PAF) Effect of antiplatelet agents on the release of PAF from human platelets

The effect of various antiplatelet agents on the release of platelet-activating factor (PAF) from human platelets was studied. The procedure for preparation of PAF was done according to the method of Cignard et al (1979). Bioassay of PAF activity was accomplished by measuring PAF-induced aggregation...

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Bibliographic Details
Published inBlood & Vessel Vol. 15; no. 5; pp. 507 - 509
Main Authors MURASHIMA, Seiko, DEGUCHI, Katsumi, NAGANO, Tadashi, TOMEOKU, Makoto, TSUDA, Masayuki, WADA, Hideo, ITO, Tadashu, KUTO, Makoto, SHIRAKAWA, Shigeru
Format Journal Article
LanguageEnglish
Published The Japanese Society on Thrombosis and Hemostasis 01.10.1984
Subjects
A 23187
aspirin
human platelet
indomethacin
PAF
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Summary:The effect of various antiplatelet agents on the release of platelet-activating factor (PAF) from human platelets was studied. The procedure for preparation of PAF was done according to the method of Cignard et al (1979). Bioassay of PAF activity was accomplished by measuring PAF-induced aggregation of gelfiltered rabbit platelets. In the absence of antiplatelet agents, PAF was released from human platelets challenged with A23187 (2.4±2.6ng/2×109 platelets at 2.5μM A23187 and 4.5±3.3ng/2×109 platelets at 5.0μM A23187). This release of PAF was not inhibited by aspirin nor indomethacin, but reduced by dipyridamole and ticlopidine. These results suggest that PAF may be the mediator of cyclooxygenase independent aggregation.
ISSN:0386-9717
1884-2372
DOI:10.2491/jjsth1970.15.507