Estimation of S-haplotypes in Yamato-mana (Brassica rapa L. Oleifera Group) grown in different seed-production fields

• Published in: Engeigaku kenkyuu Vol. 7; no. 4; pp. 505 - 510
• Main Authors: Asao, H.(Nara-ken. Agricultural Experiment Station, Kashihara (Japan)), Okuyama, E, Yano, K, Nishimoto, T, Hojo, M, Ochi, Y, Kajita, S, Takayama, S
• Format: Journal Article
• Language: Japanese
• Published: THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE 2008
• Subjects: AUTOESTERILIDAD; AUTOSTERILITE; BRASSICA CAMPESTRIS; CLONACION; CLONAGE; CLONING; DIHAPLOIDIA; DIHAPLOIDIE; DIHAPLOIDY; F1; PCR; SELF STERILITY; SLG; SP11
• ISSN: 1347-2658; 1880-3571
• DOI: 10.2503/hrj.7.505

Nara prefecture certifies Yamato-mana (Brassica rapa L. Oleifera Group) as the traditional vegetable of the Yamato District, and is providing support for its cultivation and marketing. However, Yamato-mana has not yet been widely distributed and sold because the harvested product is not uniformly sized and its outer leaflets are likely to turn yellow soon after harvesting. Therefore, to overcome these problems, efforts are underway to breed an Fsub(1) hybrid of Yamato-mana using its nature of strong self-incompatibility. To estimate S-haplotypes in six varieties of Yamato-mana sampled in different locations in Nara prefecture, we cloned class-I SLG and class-II SP11 from these varieties by PCR. In total, 31 genes were confirmed from sequences of amplified fragments, including 27 class-I S-haplotypes and 4 class-II S-haplotypes. Cluster analyses, based on S-haplotypes accumulated in each variety, classified the six varieties into three groups: Nara prefectural agricultural experiment station/Nanto varieties, Takada/Gojo/Fukase varieties, and Yamato agricultural farm variety.