Comparative quantification of dactylorhin among different wild tissues and in vitro cultures of Dactylorhiza hatagirea (D.Don) Soó, an endangered medicinal orchid of Western Himalayas

• Published in: South African journal of botany Vol. 153; pp. 172 - 177
• Main Authors: Sood, Archit, Thakur, Kanika, Joshi, Robin, Kumar, Dinesh, Warghat, Ashish R
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.02.2023
• Subjects: Dactylorhin; Dactylorhiza; In vitro; Quantification; Dactylorhin; Quantification; Dactylorhiza; In vitro
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2022.12.024

Dactylorhiza hatagirea, a terrestrial orchid endemic to North-Western Himalayas has immense medicinal importance attributed to dactylorhin. Inspite of therapeutic relevance, quantification of dactylorhin from this orchid has not been reported so far. Also, there is tremendous need to conserve D. hatagirea through in vitro approaches to harness its various bio-constituents. In the current study, it was found that flowers/inflorescences were enriched with dactylorhin along with tubers in D. hatagirea. Other aerial parts such as stem and leaves were not contributing towards the accumulation of active secondary metabolites collectively in whole plant. Also, the protocol for regeneration of multiple shoots with tubers/roots and callus initiation in D. hatagirea was optimized. BM2 terrestrial orchid media supplemented with 1 mg/L indole-3-butyric acid (IBA) and 6 mg/L 6-Benzylaminopurine (BAP) promoted shoot and root regeneration rapidly and efficiently. Moreover, Murashige Skoog (MS) media containing 0.5 mg/L 1-Naphthaleneacetic acid (NAA) and 1 mg/L BAP was suitable with a high frequency rate and a shorter duration for callus initiation. Further, it was observed that there was less enrichment of dactylorhins in in vitro cultures compared to naturally grown plant parts, but can be exploited alternatively if needed. [Display omitted] •In vitro protocol for shoot/root regeneration and callus initiation was standardized in D. hatagirea.•Detailed quantification of dactylorhins was performed in D. hatagirea.•Dactylorhins were more accumulated in tubers along with floral parts.•Alternative production of dactylorhins in vitro cultures was reported.