Some peculiarities of the outer membrane composition of marine Gram-negative bacterium Chryseobacterium indoltheticum CIP 103168T

Some peculiarities of the lipid composition of the outer membrane of the marine Gram-negative bacterium Chryseobacterium indoltheticum CIP 103168T have been studied. It is found that C. indoltheticum cells do not contain phosphatidylglycerol. Lipopolysaccharide could not be extracted by conventional...

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Published inBiochemistry (Moscow). Supplement series A, Membrane and cell biology Vol. 1; no. 2; pp. 114 - 122
Main Authors Vorob’eva, E. V., Krasikova, I. N., Dmitrenok, A. S., Dmitrenok, P. S., Drozdov, A. L., Reunov, A. V., Lapshina, L. A., Solov’eva, T. F.
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.06.2007
Subjects
Acetic acid
Bacteria
Chloroform
Chryseobacterium
Composition
Glucosamine
Gram-negative bacteria
Lipid A
Lipid composition
Lipids
Lipopolysaccharides
Membrane composition
Membranes
Monosaccharides
Phosphatidylglycerol
Polysaccharides
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Summary:Some peculiarities of the lipid composition of the outer membrane of the marine Gram-negative bacterium Chryseobacterium indoltheticum CIP 103168T have been studied. It is found that C. indoltheticum cells do not contain phosphatidylglycerol. Lipopolysaccharide could not be extracted by conventional methods; therefore, lipid A was isolated by hydrolysis of whole bacterial cells with 10% acetic acid with subsequent treatment with a chloroform: methanol mixture. The isolated lipid A has an unusual monosaccharide structure and constitutes 1-phosphate-D-glucosamine acylated with (R)-3-hydroxy-15-methylhexadecanoic and (R)-3-hydroxy-13-methyltetradecanoic acids at C2 and C3 atoms, respectively. The C. indoltheticum cells are shown to have a capsule; its polysaccharide apparently fulfils the LPS function.
ISSN:1990-7478
1990-7494
DOI:10.1134/S1990747807020031