Stochastic Modeling of S. cerevisiae Inactivation by Supercritical CO 2
Abstract Pasteurization of S. cerevisiae in a simple substrate with supercritical CO 2 was performed at 36 °C on a laboratory multibatch apparatus of a total volume of 150 mL. The pressure values ranged from 100 to 300 bar. The results show a clear dependence between inactivation ratio and increase...
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| Published in | Biotechnology progress Vol. 21; no. 5; pp. 1461 - 1465 |
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| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
01.09.2005
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| Online Access | Get full text |
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| Summary: | Abstract
Pasteurization of
S. cerevisiae
in a simple substrate with supercritical CO
2
was performed at 36 °C on a laboratory multibatch apparatus of a total volume of 150 mL. The pressure values ranged from 100 to 300 bar. The results show a clear dependence between inactivation ratio and increase of pressure. A mathematical modeling of the process was exploited to fit the experimental evidences: inactivation curves were analyzed using a stochastic model based on the multihit model (
1
). The nonlinear survival curve shows a shoulder and a tail which represent the lag and the resistant phase, respectively. The meaning of the nonlinear relationship between inactivation ratio and time is also discussed; the effect of pressure on the values assumed by the parameters of the model proposed was investigated. |
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| ISSN: | 8756-7938 1520-6033 |
| DOI: | 10.1021/bp049554m |