Immunomodulatory effect of Red Moringa oleifera Leaves Fermentation Extract on Il-21 And Il-22 Expressions in Balb/C Mice Exposed to Salmonella typhi

• Published in: Research journal of pharmacy and technology Vol. 16; no. 10; pp. 4774 - 4780
• Main Authors: Estri Wuryandari, MM. Riyaniarti, Yuliati, Ninis, sutikno, Ekawati, Tuna, Hartati, Damayanti, Nita, Rifa’i, Muhaimin, Fitri Atho’illah, Mochammad, Dzariyani Laili, Rizky
• Format: Journal Article
• Language: English
• Published: Raipur A&V Publications 01.10.2023
• Subjects: Animals; Cloning; Cytokines; Fermentation; Flow cytometry; Food contamination & poisoning; Leaves; Salmonella; Tropical diseases; United States > US
• ISSN: 0974-3618; 0974-360X; 0974-306X
• DOI: 10.52711/0974-360X.2023.00774

Interleukin-21(IL-21) and interleukin-22(IL-22) expressions in the substantial intestine increase when inflammation occurs. They can also induce the production of pro-inflammatory cytokines and Matrix Metallo Proteinases (MMP) in subepithelial fibroblasts. To evaluate at IL-21 and IL-22 expressions in BALB/c mice after administering red M. oleifera leaf fermented extracts exposed to Salmonella typhi. The expression of IL-21 and IL-22 were evaluated for immunomodulatory effect was analyzed by flowcytometer. Data were analyzed by one-way ANOVA and continued with the Tukey test (p<0.05). The results showed that the fermented extract of red M. oleifera leaves could act as an immunosuppressor characterized by decreased levels of IL-21 and IL-22 both on CD4 T and CD8 T cells in mice injected with Salmonella typhi ranging from 14 mg/Kg BW to 42 mg/Kg BW and Pyrex analysis explains that the cathecin compound has the same active side as 8MR in forming bonds with MMP-9. The results of this study provide is the fermented extract of red M. oleifera leaves decreasing the expression of CD4+IL21+, CD8+IL21+ and CD4+IL22+, CD8+IL22+ through inhibition of MMP-9