Development and Validation of a New HPLC Method for the Estimation of Sulfamoxole in Bulk and Tablet Formulations

To quantify the sulfamoxole content in bulk as well as tablet formulation, a new isocratic RP-HPLC method was developed and then validated. Kromasil C18 column with a dimension of 250 X 4.6mm was used which was filled with a particle size of 5μ. A mixture containing CH3OH, CH3CN and H2O in the volum...

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Bibliographic Details
Published inResearch journal of pharmacy and technology Vol. 16; no. 12; pp. 5617 - 5623
Main Authors Karuna Moparthy, Kamala, Nadh Ratnakaram, Venkata, Prasad Gorumutchu, Giri
Format Journal Article
LanguageEnglish
Published Raipur A&V Publications 01.12.2023
Subjects
Accuracy
Acids
Chromatography
Drug dosages
Methods
Retention
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Summary:To quantify the sulfamoxole content in bulk as well as tablet formulation, a new isocratic RP-HPLC method was developed and then validated. Kromasil C18 column with a dimension of 250 X 4.6mm was used which was filled with a particle size of 5μ. A mixture containing CH3OH, CH3CN and H2O in the volume ratio of 55, 30 and 15 was used as a mobile phase at room temperature with an optimized flow rate of 1ml/min. UV detector was set at 241nm for sulfamoxole determination. The run time of the current method is ten minutes. Accomplished the forced degradation studies to understand the stability indicating nature of the current method. As per the current ICH guides (Q2R1), validation of the method was conducted.
ISSN:0974-3618
0974-360X
0974-306X
DOI:10.52711/0974-360X.2023.00908