A molecular genetic approach to roebuck individual identification in the case of poaching in Serbia
Application of the molecular genetic methods in forensic cases dealing with wild animals has significantly increased recently. These techniques are practically used in order to help solving four key problems : determination of kind of the wild animal, geographic origin, kinship ties and individual i...
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Published in | Veterinarski glasnik Vol. 67; no. 3-4; pp. 279 - 287 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Faculty of Veterinary Medicine, Belgrade
2013
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Subjects | |
Online Access | Get full text |
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Summary: | Application of the molecular genetic methods in forensic cases dealing with
wild animals has significantly increased recently. These techniques are
practically used in order to help solving four key problems : determination
of kind of the wild animal, geographic origin, kinship ties and individual
identification. In this work the first case of introducing the examination of
polimorphism of microsatelite genetic markers within forensic analysis in the
cases of poaching in Serbia is presented. The objectives of this forensic
analysis was to determine if the meat confiscated during house search of the
suspect comes from roebuck origin (Capreolus capreolus), which remains had
been found by a game warden in the field during closed season, where the
suspect denied the offense, claiming that the meat comes from other roebuck
that had been shot during the previous hunting season. DNK was isolated from
the skin and fur samples taken from the roebuck corpse found in the woods, as
well as from the frozen meat found in the suspect?s house. Both amplification
and polimorphism examination of the eight microsatelite markers (ROE01,
NVHRT21, NVHRT24, NVHRT48, NVHRT73, RT7 AND RT27) were carried out. In all
the examined samples, the same pattern of variability of the tested
microsatelites was determined, that is it was proved that DNK profiles of the
samples taken from roebuck corpse were identical to DNK profile of the meat
sample found in the suspect?s house. This result clearly indicates that all
the examined biological samples originate from the same animal, and
consequently represents forensically valid evidence in the case of roebuck
poaching. |
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ISSN: | 0350-2457 2406-0771 |
DOI: | 10.2298/VETGL1304279D |