Jag1 insufficiency alters liver fibrosis via T cell and hepatocyte differentiation defects

• Published in: EMBO molecular medicine
• Main Authors: Mašek, Jan, Filipovic, Iva, Van Hul, Noémi, Belicová, Lenka, Jiroušková, Markéta, Oliveira, Daniel V, Frontino, Anna Maria, Hankeova, Simona, He, Jingyan, Turetti, Fabio, Iqbal, Afshan, Červenka, Igor, Sarnová, Lenka, Verboven, Elisabeth, Brabec, Tomáš, Björkström, Niklas K, Gregor, Martin, Dobeš, Jan, Andersson, Emma R
• Format: Journal Article
• Language: English
• Published: Germany 02.10.2024
• Subjects: Jagged1; Alagille syndrome; Fibrosis; Notch; Treg
• ISSN: 1757-4684; 1757-4684
• DOI: 10.1038/s44321-024-00145-8

Fibrosis contributes to tissue repair, but excessive fibrosis disrupts organ function. Alagille syndrome (ALGS, caused by mutations in JAGGED1) results in liver disease and characteristic fibrosis. Here, we show that Jag1 mice, a model for ALGS, recapitulate ALGS-like fibrosis. Single-cell RNA-seq and multi-color flow cytometry of the liver revealed immature hepatocytes and paradoxically low intrahepatic T cell infiltration despite cholestasis in Jag1 mice. Thymic and splenic regulatory T cells (Tregs) were enriched and Jag1 lymphocyte immune and fibrotic capacity was tested with adoptive transfer into Rag1 mice, challenged with dextran sulfate sodium (DSS) or bile duct ligation (BDL). Transplanted Jag1 lymphocytes were less inflammatory with fewer activated T cells than Jag1 lymphocytes in response to DSS. Cholestasis induced by BDL in Rag1 mice with Jag1 lymphocytes resulted in periportal Treg accumulation and three-fold less periportal fibrosis than in Rag1 mice with Jag1 lymphocytes. Finally, the Jag1 hepatocyte expression profile and Treg overrepresentation were corroborated in patients' liver samples. Jag1-dependent hepatic and immune defects thus interact to determine the fibrotic process in ALGS.