Biodegradation of sugarcane bagasse biomass using recombinant alpha-galactosidase overexpressing whole-cell E.coli: a sustainable method of agricultural waste utilization
Whole-cell bacteria overexpressing a combo of enzymes capable of breaking down complex lignocellulosic components of cell wall is a path-breaking innovation that is eco-friendly for agricultural waste processing and sustainable environment. In this study, a whole-cell E. coli overexpressing the enzy...
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Published in | 3 Biotech Vol. 14; no. 10; p. 246 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Cham
Springer International Publishing
01.10.2024
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Subjects | |
Online Access | Get full text |
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Summary: | Whole-cell bacteria overexpressing a combo of enzymes capable of breaking down complex lignocellulosic components of cell wall is a path-breaking innovation that is eco-friendly for agricultural waste processing and sustainable environment. In this study, a whole-cell
E. coli
overexpressing the enzyme alpha-galactosidase is used to biodegrade sugarcane bagasse, presenting a sustainable approach for agricultural waste utilization. Alpha-galactosidase is an enzyme that breaks down alpha-D-galactose residues at the non-reducing ends of oligosaccharides (such as raffinose, stachyose, and verbascose), complex galactomannans, and galactolipids. Submerged and solid-state fermentation-mediated hydrolysis of bagasse waste using recombinant
E. coli
overexpressing α-galactosidase shows a decrease in the level of α-galactosides releasing sucrose and reducing sugars, indicating a continuous breakdown of the cell wall. Scanning electron microscopy indicates substantial disintegration of cell wall fibers under both submerged (12 h) and solid-state (7 days) fermentation, confirming the disruption of bagasse cell wall structural integrity. The 2XM9 media was found competent for both total protein and enzyme activity; the total protein concentration was 2553 µg/ml after 28 h of induction with an enzyme activity of 0.445 gal units/µg of protein after 16 h of induction at 24 °C. The results show that using whole-cell recombinant systems that express different cell wall-degrading enzymes could be a sustainable way to use agricultural waste, which would help with both waste management and protecting the environment. |
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Bibliography: | ObjectType-Case Study-2 SourceType-Scholarly Journals-1 ObjectType-Feature-4 content type line 23 ObjectType-Report-1 ObjectType-Article-3 |
ISSN: | 2190-572X 2190-5738 |
DOI: | 10.1007/s13205-024-04092-6 |