Comparing the effects of different exogenous hormone combinations on seed-derived callus induction in Nicotiana tabacum

Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid – IAA; 2,4-Dichlorophenoxyacetic acid – 2,4-D; kinetin – KIN; 6-Benzylaminopurine – BAP) combinations to compare different approaches to callus induction directly from...

Full description

Saved in:
Bibliographic Details
Published inActa biochimica polonica
Main Authors Lang, Jakub, Pankowski, Jędrzej, Grabarz, Piotr, Pluciński, Bartosz, Jedynak, Paweł
Format Journal Article
LanguageEnglish
Published 27.11.2020
Online AccessGet full text

Cover

More Information
Summary:Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid – IAA; 2,4-Dichlorophenoxyacetic acid – 2,4-D; kinetin – KIN; 6-Benzylaminopurine – BAP) combinations to compare different approaches to callus induction directly from the seeds of Nicotiana tabacum. Callus formation was observed up to 4 weeks after sowing and the most effective were 0.5 mg/L of 2,4-D with 0.25 mg/L of BAP and 2 mg/L 2,4-D with 1 mg/L of BAP. The calli were green, photosynthetically active and after 6 weeks of growth, no stress symptoms (estimated on the basis of fluorescence of chlorophyll a in photosystem II) were noticed.
ISSN:0001-527X
1734-154X
DOI:10.18388/abp.2020_5456