Electrochemiluminescence biosensor for hyaluronidase activity detection and inhibitor assay based on the electrostatic interaction between hyaluronic acid and Ru(bpy)32

•An ECL biosensor for hyaluronidase detection based on the electrostatic interaction was developed for the first time.•The developed sensor was also used to detect HAase inhibitor with high efficiency.•The limit of detection for hyaluronidase was 0.33 U/mL. The development of simple and sensitive me...

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Bibliographic Details
Published inSensors and actuators. B, Chemical Vol. 275; pp. 409 - 414
Main Authors Li, Zhixin, Chen, Huixing, Zhuo, Zesheng, Huang, Da, Luo, Fang, Chen, Li, Wang, Jian, Guo, Longhua, Qiu, Bin, Lin, Zhenyu
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.12.2018
Subjects
Electrochemiluminescence
Hyaluronic acid
Hyaluronidase
Ru(bpy)32
Hyaluronidase
Ru(bpy)32
Electrochemiluminescence
Hyaluronic acid
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Summary:•An ECL biosensor for hyaluronidase detection based on the electrostatic interaction was developed for the first time.•The developed sensor was also used to detect HAase inhibitor with high efficiency.•The limit of detection for hyaluronidase was 0.33 U/mL. The development of simple and sensitive method for hyaluronidase (HAase) detection is significant for the clinical diagnosis and treatment of cancer as HAase is a potential tumor marker. In this study, a simple but sensitive electrochemiluminescence (ECL) biosensor has been designed for HAase detection based on the electrostatic interaction of anionic hyaluronic acid (HA) and cationic luminous reagent Ru(bpy)32+. Ru(bpy)32+ is adsorbed on HA through electrostatic interaction to form HA-Ru(bpy)32+ complex. The Ru(bpy)32+ in the HA-Ru(bpy)32+ complex cannot be filtered out because of its large molecular weight. In the presence of HAase, the HA in HA-Ru(bpy)32+ can be cleaved into fragments by enzymatic hydrolysis. As a result, the Ru(bpy)32+ attached on the HA fragments can be separated from the HA-Ru(bpy)32+ complex by centrifugation easily. The resulting ultrafiltrate containing Ru(bpy)32+ can be used to characterize the concentration of HAase in the sample. The ECL intensity of Ru(bpy)32+ has a linear relationship with the concentration of HAase in the range of 2.0–40 U/mL with a detection limit of 0.33 U/mL. The proposed ECL system has been applied to detect HAase in urine samples with satisfied results and HAase inhibitor with high efficiency.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2018.08.069