Response of Prokaryotic Communities to Freshwater Salinization

• Published in: Applied Microbiology (Basel) Vol. 2; no. 2; pp. 330 - 346
• Main Authors: Gagnon, Jean-Christophe, Astorg, Louis, Derry, Alison M., Lazar, Cassandre Sara
• Format: Journal Article
• Language: English
• Published: 19.05.2022
• ISSN: 2673-8007; 2673-8007
• DOI: 10.3390/applmicrobiol2020025

Each year, millions of tons of sodium chloride are dumped on roads, contributing to the salinization of freshwater environments. Thus, we sought to understand the effect of sodium chloride (NaCl) on freshwater lake prokaryotic communities, an important and understudied component of food webs. Using mesocosms with 0.01–2.74 ppt NaCl (0.27–1110.86 mg/L Cl−), we evaluated the effect generated on the diversity and absolute abundance of prokaryotic populations after three and six weeks. A positive relationship between Cl− values and absolute bacterial abundance was found after three weeks. The influence of eukaryotic diversity variation was observed as well. Significant differentiation of bacterial communities starting at 420 mg/L Cl− was observed after three weeks, levels lower than the Canadian and US recommendations for acute chloride exposure. The partial recovery of a “pre-disturbance” community was observed following a drop in salinity at the threshold level of 420 mg/L Cl−. A gradual transition of dominance from Betaproteobacteria and Actinobacteria to Bacteroidia and Alphaproteobacteria was observed and is overall similar to the natural transition observed in estuaries.