The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extr...

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Bibliographic Details
Published inIndonesian Journal of Medical Laboratory Science and Technology Vol. 3; no. 1; pp. 56 - 63
Main Authors Puspita, Julia Nanda, Kurniatuhadi, Rikhsan, Rahmawati, Rahmawati
Format Journal Article
LanguageEnglish
Published Universitas Nahdlatul Ulama Surabaya 28.04.2021
Subjects
Antibacterial
Escherichia coli
Staphylococcus aureus
Thermoactinomyces sp. (H24)
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Summary:Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).
ISSN:2684-6748
2656-9825
DOI:10.33086/ijmlst.v3i1.1700