A Strategic Production Improvement of Streptomyces Beta Glucanase Enzymes with Aid of Codon Optimization and Heterologous Expression

β-glucan rich cereals such as barley and oats serve as a raw material in breweries and also used as an animal feed. Digestion of β-glucan is often a major hurdle, thus providing exogenous enzyme β-glucanase serves as an option. The present study takes an effort for the expression and over production...

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Published inBiosciences, biotechnology research Asia Vol. 17; no. 3; pp. 587 - 599
Main Authors Edison, Lekshmi K., Dan, Vipin Mohan, S. R, Reji, N. S, Pradeep
Format Journal Article
LanguageEnglish
Published Bhopal Biosciences Biotechnology Research Asia 20.09.2020
Subjects
Animal feed
Barley
Breweries
Cereals
Chemical properties
Chromatography
Cloning
E coli
Enzymes
Gene amplification
Genes
Glucan
Optimization
Protein expression
Proteins
Streptomyces
Substrates
Thermal stability
β-Glucan
United States > US
Western Ghats
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Summary:β-glucan rich cereals such as barley and oats serve as a raw material in breweries and also used as an animal feed. Digestion of β-glucan is often a major hurdle, thus providing exogenous enzyme β-glucanase serves as an option. The present study takes an effort for the expression and over production of β-glucanase genes from Streptomyces sp in E. coli. The exo-β-1,4-glucanase and endo-β-1,3-glucanase encoding genes were isolated and codon optimized and significant-high expression levels were obtained in E. coli strain. The expressed enzymes showed broad pH stability, good thermostability, and better affinity towards the barley β-glucan substrate. The study implies that heterologous expression with codon optimization strategy enhances the production of Streptomyces origin beta-glucanase enzymes with prominent physio-chemical properties for efficient beta-glucan degradation.
ISSN:0973-1245
2456-2602
DOI:10.13005/bbra/2862