Retracted: LncRNA LINC01305 silencing inhibits cell epithelial‐mesenchymal transition in cervical cancer by inhibiting TNXB‐mediated PI3K/Akt signalling pathway

• Published in: Journal of cellular and molecular medicine Vol. 23; no. 4; pp. 2656 - 2666
• Main Authors: Yan, Shu‐Ping, Chu, Dan‐Xia, Qiu, Hai‐Feng, Xie, Ya, Wang, Chun‐Fang, Zhang, Jian‐Ying, Li, Wen‐Cai, Guo, Rui‐Xia
• Format: Journal Article
• Language: English
• Published: Chichester John Wiley & Sons, Inc 01.04.2019
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Bioinformatics; Cell migration; Cervical cancer; Cervix; E-cadherin; Females; Gelatinase A; Gelatinase B; Mesenchyme; Migration; Polymerase chain reaction; Reporter gene; Reverse transcription; Signal transduction; Tenascin; Therapeutic applications; Vimentin
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.14161

Abstract Cervical cancer (CC) remains one of the leading malignancies afflicting females worldwide, with its aetiology associated with long‐term papillomavirus infection. Recent studies have shifted their focus and research attention to the relationship between long non‐coding RNAs (lncRNAs) and CC therapeutic. Thus, the aim of the current study was to investigate the underlying mechanism of lncRNA LINC01305 on the cell invasion, migration and epithelial‐mesenchymal transition (EMT) of CC cells via modulation of the PI3K/Akt signalling pathway by targeting tenascin‐X B (TNXB). The expressions of LINC01305, TNXB, MMP2, MMP9, E‐cadherin, vimentin, PI3K, Akt, p‐PI3K, p‐Akt and TNXB were detected in this study. After which, the cell invasion and migration abilities of the CC cells were determined respectively. Bioinformatics and the application of a dual luciferase reporter gene assay provided verification indicating that TNXB is the target gene of lncRNA LINC01305. Reverse transcription quantitative polymerase chain reaction (RT‐qPCR) and western blot analysis methods revealed that the expressions of MMP2, MMP9, vimentin, PI3K, Akt, p‐PI3K and p‐Akt were decreased following the down‐regulation of LncRNA LINC01305 or overexpression of TNXB. LncRNA LINC01305 silencing or TNXB overexpression was noted to decrease the migration and invasion of SiHa cells. Taken together, the key findings of the current study present evidence suggesting that lncRNA LINC01305 silencing suppresses EMT, invasion and migration via repressing the PI3K/Akt signalling pathway by means of targeting TNXB in CC cells, which ultimately provides novel insight and identification of potential therapeutic targets for CC.