Expression of TGF-β2 mRNA and PCNA, FN Protein in Lens Epithelial Cells in Age-related Nuclear and Cortex Cataract

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 25; no. 5; pp. 578 - 581
• Main Author: 叶琳 李琴 蔡小军 邓平
• Format: Journal Article
• Language: English
• Published: Department of Ophthalmology, Zhongnan Hospital, Wuhan University, Wuhan 430071,China 01.09.2005
• Subjects: FN蛋白质; mRNA; PCNA; TGF-β2; 上皮细胞; 基因表达; 白内障; cortex; PCNA; cataract; FN; TGF-β2; LECs; nuclear
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/BF02896023

Summary： By using RT PCR and immunohistochemistry, the expressions of transforming growth factor 132 （TGF-β2） mRNA, proliferating cell nuclear antigen （PCNA） and fibronection （FN） protein in lens epithelial cells （LECs） of age-related nuclear and cortex cataract were detected and compared. The results of RT-PCR revealed that the expression of TGF-β2 mRNA was higher in cortex cataract than in nuclear cataract. Immunohistochemistry demonstrated that the expression of PCNA protein was lower and the expression of FN protein was higher in cortex cataract than in nuclear cataract. It was suggested that TGF-β2 , PCNA and FN might take important parts in the process of age-related cataract. Cortex cataract was related to the transdifferentiation of LECs, and nuclear cataract to the proliferation of LECs.